Table 1 Bacteria strains or plasmids used in this study and their relevant characteristics

Strains

Ea1189

Wild type

[65]

Ea1189ΔatpB

atpB deletion mutant

This study

Ea1189ΔatpBC

Deletion of the chromosomal region that spans the atpB, atpE, atpF, atpH, atpA, atpG, atpD, and atpC genes

This study

Plasmids

pKD3

Contains FRT-flanked Cm^r cassette sites; R6K ori; Cm^r

[66]

pKD46

Contains lambda Red recombinase induced by L-Arabinose; R101 ori; Ap^r

[66]

pEVS143

Broad-host-range cloning vector; IPTG inducible Cm^r; pES213 ori; Km^r

[67]

pOE-hok

pEVS143 cmR::hok; overexpression vector; Km^r

[27]

pBAD33

Broad-host-range cloning vector; arabinose inducible Cm^r; pACYC18 ori

[68]

pBAD33-pspA

pBAD33 cmR::pspA; overexpression vector; Cm^r

This study

pPROBE-NT

Broad-host-range promoter-probe vector; pBBR1 ori; Km^r

[69]

pPROBE-pspA

pPROBE-NT::pspA; native promoter of pspA in pPROBE-NT; Km^r

This study