Fig. 3

Mutations in the Sclerotinia sclerotiorum os1 gene likely conferred resistance to fludioxonil. a Alignment of the OS1 protein sequences of the strains F4 and F5 and their parent strain CU11.19. The top line is the consensus sequence. Each amino acid is represented in a different colour. The blue bars represent PFAM domains present in the OS1 protein. The grey bars represent the amino acid substitutions present in F4 and F5. b The non-synonymous nucleotide substitutions in F4 and F5 that caused missense amino acid replacements in the OS1 protein. A point mutation at position 1474 of the OS1 coding DNA sequence (CDS) from G to A in F4 caused a non-synonymous mutation from Alanine (A) to Threonine (T). A point mutation at position 1756 of the OS1 CDS from G to A in F5 caused a non-synonymous mutation from Glycine (G) to Arginine (R)