Table 1 Strains and plasmids used in this study
From: High-pressure processing-induced transcriptome response during recovery of Listeria monocytogenes
Plasmids and strains | Characteristics | Source/ Reference |
|---|---|---|
Plasmids | ||
 pORI280 | RepA− gene replacement vector, constitutive lacZ, 5.3 kb, Emr | [97] |
 pORI280ADΔlmo1013 | pORI280 containing 503-bp region on either side flanking lmo1013 deletion pORI280 including both upstream and downstream flanking regions (503 bp each) of lmo1013 deletion | This study |
 pORI280ADΔlmo2229 | pORI280 containing 503-bp region on either side flanking lmo2229 deletion pORI280 including both upstream and downstream flanking regions (503 bp each) of lmo2229 deletion | This study |
 pVE6007 | Temperature-sensitive helper plasmid that provides RepA in trans, Cmr | [98] |
E. coli strains | ||
 EC10B | DH10B derivative with Kanr and RepA integrated in the glgB gene | [99] |
 EC10B/pORI280ADΔlmo1013 | E. coli EC10B with pORI280ADΔlmo1013 | This study |
 EC10B/pORI280ADΔlmo2229 | E. coli EC10B with pORI280ADΔlmo2229 | This study |
L. monocytogenes strains | ||
 EGDe | Serotype 1/2a | |
 EGDeΔlmo1013 | EGDe with the entire lmo1013 gene deleted | This study |
 EGDeΔlmo2229 | EGDe with the entire lmo2229 gene deleted | This study |