Fig. 4
a + b Northern blot analysis of the sRNAs CcsR1, SorY, UpsM, IGR_1711_rpsL and 5′ UTR RSP_6083. Total RNA was isolated during exponential growth phase (OD660 = 0.45) from microaerobic cultures of wild type or pnp mutant. Loading control: 5S rRNA. The barchart illustrates the log2−fold changes within the indicated sRNAs. n = 3. c Wild type and pnp mutant cells were cultivated under microaerobic conditions until exponential phase. Samples were harvested before (t0) and after (t5-t30) addition of 0.2 mg/ml rifampicin. Total RNA was isolated and used for Northern blot analysis. Loading control: 5S rRNA. d The signal intensities for the indicated probes were normalized to the 5S rRNA signal, timepoint t0 was set to 1. x-axis: minutes after addition of 0.2 mg/ml rifampicin; y-axis: relative signal intensity. Solid lines represent mean value for biological triplicates, light colors indicate the standard deviation (n = 3). Blue: pnp mutant; red: wild type. The full-length Northern blots are presented in Supplementary Fig. S4, S5, S6 and S7, Additional file 1