Fig. 6
Many differential 3′ ends found in the PNPase mutant can also be detected in the RNase III or RNase E mutant strain at the same genomic position. a, d, g Venn diagrams illustrating the total number of differential 3′ ends found in each mutant strain in comparison to the wild type (+: mutant enriched; −: mutant depleted) and the number differential 3′ ends which could be mapped to the same genomic position. Percentage: number of 3′ ends in respective quadrant devided by the total number of rnc/rne 32 °C/rne 42 °C enriched or depleted 3′ ends. Blue: pnp mutant; violett: rnc mutant; yellow: rne mutant at 32 °C; red: rne mutant at 42 °C. b, e, h Scatterplots depicting the intersecting analysis, every dot represents one differential 3′ end. x-axis: log2-fold change pnp mutant versus wild type; y-axis: log2-fold change rnc/rne mutant versus wild type. Feature classes harboring the observed 3′ ends are color coded. Orange: CDS; yellow: ncRNA; green: rRNA; blue: tRNA; violett: UTR. Blue lines represent a 2D density function. The histograms (grey) illustrate the distribution of intersecting 3′ end log2-fold changes of the compared strains. d, f, i Histograms display the number of intersecting 3′ ends, which are grouped in mutant enriched/depleted and feature classes. x-axis: feature class; y-axis: count