Fig. 2

Volcano plot and cluster analysis of differentially expressed genes (DEGs). a Volcano plot. Red dots (UP) represent significantly upregulated genes (log₂FC ≥ 1.0, FDR < 0.05); blue dots (DOWN) represent significantly downregulated genes (log₂ FC ≤ − 1.0, FDR < 0.05); and black dots (NO) represent DEGs below the level of significance; (b) based on 3486 known DEGs in DIMFPs and DAFPs of chickens, cluster analysis was performed. The results show that the gene expression profiling data in the same group were closely related