Fig. 4

DE mRNAs in PAMs upon PRRSV infection. a Volcano plots of mRNAs between mock and PRRSV-infected PAMs. Red or green points represent the DE mRNAs with statistical significance (P < 0.05). b Unsupervised hierarchical clustering and heat map of mRNA expression in PAMs upon virus infection. Red color indicates upregulated expression and blue color indicates downregulated expression. Each column represents a PAM sample and each row represents an mRNA fragment. Mock represents mock-treated PAMs and virus represents PRRSV-infected cells. c and d Pathway analysis of DE mRNAs after PRRSV infection. The dot plots present the enrichment of the mRNAs in each pathway. Pathway enrichment of upregulated mRNAs (c) and downregulated mRNAs (d) was shown respectively