Fig. 1

Design of a genome-wide yeast CRISPRi library. a Candidate guide RNA sites in promoter regions were identified and scored to prioritize target sites that were unique in the genome, specific to one promoter, and located in accessible chromatin. b Promoter regions targeted by guide RNAs. When transcription start sites were known, we selected one guide each from three separate regions around the transcription start site, along with seven others in the overall promoter. When transcription start sites were not known, we targeted a wider area upstream of the start of the coding sequence. c Cumulative distribution of genes targeted by up to ten distinct guides. Unambiguous guides do not fall within the potentially active region for any other gene. Assigned guides have a single likely target based on empirical measurements of guide activity and location. d Schematic of an ambiguous guide at a divergent promoter