Fig. 5

Phylogenetic analysis supports orthology relationships. Twelve candidate gene families (a-l), where BUSCO failed to detect orthologs in diplogastrid genomes, were selected as candidates for phylogenetic analysis. Maximum-likelihood trees were reconstructed from BUSCO orthologs of other nematode species as well as diplogastrid sequences, which were identified as best-reciprocal hits against C. elegans proteins. Stars denote branches with a bootstrap support ≥99/100. In most cases, the phylogenies are largely consistent with the species tree and the diplogastrid sequences form a monophyletic clade that correctly groups as sister to the rhabditids (C. elegans and H. contortus)