Fig. 5

Frequency of AAG codon in different groups of proteins under short-term heat stress. a Distribution of AAG codon occurrence frequency for different groups of genes in yeast strain BY4742 subjected to short-term heat stress. The proteome data used in this analysis was published by the previous study [40]. Yeast was cultured at 30 °C was taken as control. Heat stress treatment was applied by transferring yeast to 37 °C for 1 h. Up and down-regulated proteins in response to heat treatment were identified with criteria “FDR < 0.05 and fold change >1.2 or < 0.83”. The Y-axis represents the frequency of codon AAG in protein-coding sequence. P-values were calculated by the Kruskal-Wallis method. b Distribution of AAG codon occurrence frequency for different groups of genes in yeast strain BY4741 subjected to short-term heat stress. The proteome data used in this analysis was published by the previous study [41]. Yeast strain BY4741 cultured at 25 °C was taken as control. For heat treatment, yeast was exposed to 37 °C for 5, 10, 15, 30, 45 and 60 min. The criteria for up-regulated and down-regulated proteins are identified by adjusted p < 0.01 and fold change >1.2 or < 0.83. The numbers in brackets represent the heat treatment time