Fig. 8

The subcellualr localization of SmABCG46. Confocal laser scanning microscopy images showed tabacco leaf epidermal cells transiently expressing either SmABCG46::GFP (a-d) or GFP (e-h) together with PM-YFP (plasma membrane marker, Chen et al. [80]). GFP was fused to a downstream of the CDS sequence corresponding to the SmABCG46 without stop codon and transiently expressed in tobacco leaf. GFP fluorescence indicates the location of fusion protein (shown in green). The location of plasma membrane was determined by the fluorescence of PM-YFP. Column labelled “Merged” represents all the combined fluorescent signals. a/e Confocal images via the GFP channel only. b/f Confocal images of the YFP fluorescence marking with PM position. c/g Merged images of GFP (green) and YFP (yellow) together with bright field. d/h Bright field. Scale bar, 25 μm. The measurement resolution of the acquired image was 1024 × 1024