Table 14 Oligonucleotides primer-probes designed for the ten uni-genes of interest amplification and validation. *fwd = forward strand, rev = reverse strand
Uni-genes | Primer Sequence | Melting Temperature (0c) |
|---|---|---|
1_CL1597Contig1 (fwd) | GTG AAG TTG GTG CTC AGG TC | 59 |
1_CL1597Contig1 (rev) | GCT CTA CTG GTT GGG AAA CG | |
1_CL2788Contig1 (fwd) | TGA CGT TGT GCA CTT CTT GG | – |
1_CL2788Contig1 (rev) | GTG AAG ACG AGG CAG CAC | |
1_CL2708Contig1 (fwd) | CTT CTA GTT CGG TGT TGC GG | 56.6 |
1_CL2708Contig1 (rev) | CGA GAG AAG GGC GCG ATG | |
1_CL2412Contig1 (FoxP3) (fwd) | GGC AGA AAG CAC TT AGG TC | 56.8 |
1_CL2412Contig1(FoxP3) (rev) | CAG CCG TAT GTT CGG GTA CT | |
1_CL41Contig6 (fwd) | GGA GGA CAG TTG TAG GGA CA | 55.7 |
1_CL41Contig6 (rev) | GTT GTC ACC CAC TGC GTG | |
1_CL2624Contig1 (fwd) | TCC GCC GGC TTT AAT TCT TC | 55.7 |
1_CL2624Contig1 (rev) | GCG GCG GGG AGA ATT AAT AA | |
1_CL2766Contig1(fwd) | GCA CGT TCC CAT AGC TGT TG | – |
1_CL2766Contig1(rev) | GAG GTG GAG CTG GA GTG AT | |
1_CL1576Contig1(fwd) | GTG ATG GGT GTT GTG CTC AG | 56.8 |
1_CL1576Contig1(rev) | AAG AAG AGA AA GAG GCC GT | |
1_CL1679Contig1(fwd) | CCT GAG CCA TGA ATG ATA CGC | 56.8 |
1_CL1679Contig1(rev) | GAC GAG GTGAAGGAG TCG AA | |
1_CL2484Contig1(fwd) | AGG AGG AGG AGG AAG GCT AT | 57.0 |
1_CL2484Contig1 (rev) | CCC TGT GAT GAG GTT TGC AG |