Chromosome-level genome assembly and manually-curated proteome of model necrotroph Parastagonospora nodorum Sn15 reveals a genome-wide trove of candidate effector homologs, and redundancy of virulence-related functions within an accessory chromosome

Table 1 Summary of new gene annotations of P. nodorum reference isolate Sn15. A) Comparison of high-confidence Set A and low confidence Set B to previous annotation versions and B) summary of data supporting gene annotations

A) Summary	Previous studies [20, 23]		This study (Set A)			This study (Set B)
Number of genes	13,569		13,869			2534
Number of mRNAs	13,944		14,160			2557
Average gene length, bp	1558		1488			373
Number of exons	36,557		36,447			3070
Average exons/gene	2.6		2.6			1.2
Average exon length (bp)	556		539			299
Gene density (genes / Mbp)	369		372			440 (Set A + Set B)
B) Supporting Data	Loci	FPKM > 50	FPKM > 5	FPKM < 5	SignalP	SignalP+ EffectorP	Region not in previous assembly	Fuctional annotation
Set A	13,869	4033	9968	4030	1488	340	19	9505
In previous studies [20, 23]	13,663	3742	9633	4030	1463	323		9456
Not in previous studies [20, 23]	206	291	335	0	25	17		49
Set B	2534	220	2508	26	97	61	23	75
In previous study [1]	117	5	117	0	19	6		0
New or modified genes	2417	215	2391	26	77	55		75

ISSN: 1471-2164