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Table 2 Group A1 Normalized reads

From: Comparisons among rainbow trout, Oncorhynchus mykiss, populations of maternal transcript profile associated with egg viability

 

Low quality

Medium quality

High quality

    

Gene

Mean

SEM

Mean

SEM

Mean

SEM

Mean reads

RSQ

RC

P value

Mitochondrial genes

 mt-atp8

64,319

5561

90,104

10,130

108,669

14,165

92,682

0.0848

515.0

0.0717

 mt-co1

162,344

27,574

258,866

31,556

295,392

46,645

258,215

0.0255

899.0

0.2100

 mt-cytb

91,006

18,224

149,496

16,174

167,933

24,250

147,946

0.0739

799.3

0.0426

 mt-nd4l

6761

570

8704

954

9877

1308

8828

0.0663

41.9

0.1839

 mt-dlp

12,706

1006

20,170

2665

25,906

4547

21,029

0.0178

67.5

0.2258

Nuclear genes

 agfg1-like

57.4

2.9

61.6

6.7

66.8

8.2

62.7

0.0036

0.0652

0.7895

 anxa2

48.2

5.1

67.8

7.0

78.0

9.5

68.5

0.0476

0.2650

0.1173

 apoc1

468.5

247.2

1450.8

329.0

492.0

90.9

1028.4

0.0152

5.8891

0.0433

 atg16l1

39.3

6.4

62.6

6.9

70.0

10.2

62.0

0.0408

0.2489

0.0947

 bmp10-like

27.1

4.5

38.1

4.0

45.0

5.5

38.9

0.0547

0.1650

ND

 ctsz

15,381.5

2115.8

10,910.5

935.6

13,587.7

1600.5

12,306.0

0.0067

−15.5459

0.3711

 cycB

11,733.3

2287.4

9881.1

444.9

11,933.6

795.4

10,754.0

0.0001

−1.1530

0.9792

 dcaf11

80.6

8.1

140.1

11.9

153.4

16.1

136.8

0.1081

0.7006

0.0068

 dglucy

11.7

2.3

13.5

0.6

11.9

1.0

12.8

0.0040

−0.0081

ND

 erich3

14.0

2.0

13.5

0.6

12.0

1.0

13.1

0.0491

−0.0274

ND

 fbxo5

325.8

55.0

446.7

44.0

563.5

70.0

468.0

0.0512

1.8846

0.0996

 galnt3

261.8

14.6

188.2

11.5

201.6

15.7

201.6

0.0671

−0.5478

0.0321

 gsh-px

175.4

21.8

266.6

24.7

333.2

49.4

276.0

0.0684

1.3579

0.0205

 gtf3a

122.9

15.0

230.8

22.1

255.6

38.2

225.1

0.0961

1.3339

0.0041

 haus3

144.1

28.8

210.9

20.3

237.3

31.3

210.8

0.0371

0.7217

0.1045

 hbb

2926.0

1803.7

4606.3

845.1

1833.6

647.8

3529.8

0.0003

−2.4889

0.4535

 ifngr1

15.2

2.8

13.5

0.6

12.3

1.0

13.3

0.0708

−0.0359

ND

 igf-1

19.7

2.9

24.0

2.3

24.7

3.7

23.7

0.0025

0.0211

ND

 igf-2

46.2

7.8

37.1

3.8

54.2

10.7

43.6

0.0025

0.0493

ND

 igf-3

15.5

3.1

24.9

2.8

31.3

4.8

25.7

0.0587

0.1321

ND

 igfr1b

65.5

7.9

86.8

8.3

89.4

9.7

84.9

0.0209

0.1958

0.2058

 il17rd

328.9

14.3

285.6

24.2

303.5

26.6

296.6

0.0062

−0.3000

0.3318

 impa2

583.6

90.5

1790.5

180.4

2242.2

274.9

1780.8

0.2017

16.0697

<.0001

 ing3

56.7

8.8

75.7

6.5

90.0

6.9

77.8

0.0977

0.3409

0.0099

 itga7

18.2

3.7

26.6

2.9

28.4

4.5

26.1

0.0125

0.0587

ND

 kmt5b

59.2

3.4

61.0

6.0

59.3

4.9

60.2

0.0014

0.0333

0.9126

 krt18

41.9

22.6

95.5

14.7

58.2

11.6

77.1

0.0453

0.4992

0.0098

 krt8

53.6

25.4

73.0

14.0

42.7

6.4

61.1

0.0099

0.2094

0.2152

 lin7b

160.8

32.6

343.1

29.4

499.4

58.2

369.1

0.2269

3.2817

<.0001

 mettl3

11.7

2.3

13.5

0.6

11.9

1.0

12.8

0.0040

−0.0081

ND

 mr-1

281.7

41.9

248.7

10.6

275.6

16.0

261.3

0.0001

−0.0212

0.9661

 mrpl39-like

54.8

9.4

179.4

20.6

255.5

40.2

187.6

0.1605

1.8167

0.0006

 myo1b

115.4

12.2

78.8

6.5

73.8

6.4

81.8

0.1093

−0.3735

0.0108

 nasp

122.2

26.3

214.4

22.2

253.2

32.3

215.0

0.1182

1.4015

0.0014

 npm2

12.1

2.2

13.5

0.6

11.9

1.0

12.8

0.0085

−0.0117

ND

 ntan1

326.0

31.4

309.2

22.0

368.3

29.6

329.7

0.0030

0.2094

0.9207

 pde4d

58.3

8.0

79.9

9.5

89.1

12.5

80.1

0.0132

0.1851

0.3982

 pgk1

118.6

10.5

219.7

17.8

223.8

19.5

208.3

0.0992

0.9516

0.0023

 phb2

60.2

12.0

63.6

6.0

69.0

7.3

64.9

0.0109

0.1067

0.4136

 psmb9

32.5

19.5

53.4

8.3

40.7

7.5

46.8

0.0392

0.2712

0.0029

 ptgs2

505.0

113.3

424.6

28.0

518.9

44.5

464.1

0.0181

0.8142

0.4600

 pyc

64.4

4.8

68.2

6.9

71.9

8.0

68.9

0.0018

0.0461

0.8653

 ran

22.5

3.6

34.4

2.9

33.5

4.3

32.6

0.0552

0.1220

ND

 rpl22

230.7

39.3

448.7

52.3

484.2

74.6

432.5

0.0636

2.3447

0.0040

 rpl24

434.3

122.4

903.7

146.1

810.8

139.7

816.0

0.0132

2.6224

0.0473

 rpl30

563.4

180.3

1094.1

164.4

807.7

137.7

938.2

0.0136

2.9736

0.0538

 rplp1

35.0

4.2

45.4

4.7

56.3

6.8

47.5

0.0400

0.1685

0.1600

 rps9

142.3

27.2

264.5

37.4

214.9

24.7

233.7

0.0425

1.1405

0.0227

 s100a1

115.1

14.3

160.7

18.0

177.8

27.8

160.3

0.0218

0.4758

0.3086

 samm50

196.1

34.4

443.8

35.9

540.1

47.4

443.0

0.2166

3.2374

<.0001

 sec14l2

48.4

5.9

74.1

8.1

87.4

10.4

75.0

0.0572

0.3331

0.0628

 senp7

35.5

5.8

57.4

5.9

77.6

13.9

61.0

0.1255

0.4848

0.0050

 ska3

84.0

8.6

167.6

15.3

233.7

28.4

177.8

0.1846

1.4286

0.0005

 slc7a6os

169.4

35.4

285.5

25.8

364.0

42.2

295.5

0.1429

1.9438

0.0026

 smc6

110.0

8.7

86.6

6.6

91.7

7.3

91.1

0.0313

−0.1914

0.1576

 tfip11

38.8

4.4

79.1

7.2

103.8

16.0

81.8

0.1226

0.5800

0.0007

 tob1

184.5

26.8

107.1

8.1

99.0

10.7

114.2

0.2126

−0.8219

0.0004

 tubb

1071.7

186.7

1835.7

119.1

2181.3

170.6

1848.2

0.2077

10.8335

0.0001

 uchl1

177.1

19.9

272.5

21.5

294.8

21.9

267.6

0.1417

1.3368

0.0033

 vasa

479.6

35.4

377.6

28.8

377.0

27.2

390.2

0.0508

−1.0327

0.0819

  1. Low quality is 0–20% eyeing (N = 6), Medium quality is 20–80% eyeing (N = 27), High quality is 80–100% eyeing (N = 15). Values in bold are significant at P < 0.05, ND indicates below detection limit. RSQ is square root, RC is regression coefficient, and P value is for regression of transcript abundance to eyeing rate for 48 individual samples. RSQ and RC are for normalized data and P value is for transformed normalized data
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