Fig. 2

Chromatin accessibility is associated with nucleosome turnover and transcription factor binding. (A) The Western blot shows induction of an ectopic H3-3xFLAG construct under the control of a copper-regulated promoter to measure integration of new H3 (i.e. hH3 turnover). Protein levels are shown at 0, 1, 2, 3, 4, and 8 h after induction using the copper chelator BCS. (B) The genome browser image for the left arm of Linkage Group 1 (LGI-Left Arm) illustrates the overlapping patterns of accessible chromatin (blue) and newly incorporated H3-3xFLAG (green). (C) The heatmap shows global overlap of chromatin accessibility and histone turnover measured by H3-3xflag enrichment following induction. The heatmaps are centered on ACRs and ordered by peak size. (D) Representative genome browser images of the frq locus and vvd locus illustrate ATAC-seq enrichment patterns in WT and the Δwc-2 mutant. (E) The heatmap shows enrichment data across all WC2-binding sites for the indicated experiments. Previously published WC-2 ChIP-seq enrichment is shown for the wild-type strain. ATAC-seq enrichment is shown for wild type and the Δwc-2 mutant