Fig. 7From: Deploying new generation sequencing for the study of flesh color depletion in Atlantic Salmon (Salmo salar)Comparison of variations within biological replicates of 15 DEGs from the Quantseq and Trueseq libraries and RT-qPCR validation using the same biological samples; Gene expression in (A) QuantSeq library; (B) TruSeq library; (C) RT-qPCR. From left to right, the first five genes were significantly different between HN and LB in both library methods but only ldtn shows a significant difference in RT-qPCR assay; the next five genes showed significant differences only in the QuantSeq library analysis, and only two of those (fabp1 and lyama) were significantly different in RT-qPCR; the last five genes showed significant differences only in the TruSeq library, and only three out of these five genes (ovcm2, noxo1 and fclt3) were significantly different using the RT-qPCR assay. Asterisks (* and **) indicate significant difference between the HN and LB phenotypes at P < 0.05 and P < 0.01, respectivelyBack to article page