Fig. 6

Expression level of limber pine NLR genes mapped in seed family LJ-112. Relative gene expression levels were calculated as Reads per Kilobase of transcript per Million RNA-seq reads (RPKM). (a) RPKM values (y-axis) are plotted across all 386 mapped genes (x-axis) with average value from the highest to the lowest. (b) Expression profiling of NLRs mapped at the Cr4 region as well as genes with the differential expression across three seed families. Res-1: resistant seed family NR-3647; and Sus-1 and -2: susceptible seed families MRO-3501 and UT-3359A, respectively. Baggerley’s test was used to measure difference between seed families with FDR corrected p-values < 0.05 (*), < 0.01 (**), and < 0.001 (***)