Fig. 2
From: Reverse complementary matches simultaneously promote both back-splicing and exon-skipping
circRNAs are enriched in the neurons of C. elegans. a Steps to define high-confidence circRNAs used for downstream analysis. Numbers in the brackets are circRNA numbers. b Overlap of circRNAs detected in the “sort group” and the “whole group”. c Top 10 enriched gene ontology (GO) terms of parental genes of the high-confidence circRNAs. d Scheme showing amplification of back-splicing junction of a circRNA from glr-2 using divergent primers. Amplified sequences are confirmed by Sanger sequencing. The red triangle denotes the joint site. e RT-qPCR results of the fold changes of circRNAs and two linear mRNAs (pmp-3 and cdc-42, inset) after RNase R treatment. The blue dashed line shows one-fold change. Error bars are the standard deviations of three biological replicates. f, g. TPM (transcripts per million reads) comparison of all circRNAs (f) and shared circRNAs (g) between the “sort group” and the “whole group”; p values are paired Wilcoxon test. h. Scatter plot showing the fold changes of 268 circRNAs with baseMean > 3 versus fold changes of their corresponding linear mRNAs. The Pearson correlation coefficient (R) and p value (p) are shown. Significantly differentially expressed circRNAs are shown by colored dots. Names of several circRNA genes are labeled