Genome insights into the pharmaceutical and plant growth promoting features of the novel species Nocardia alni sp. nov

Table 3 Phenotypic, enzymatic and biochemical properties that distinguish strain ncl2^T from Nocardia vaccinii DSM 43285^T

Characteristics	Isolate ncl2^T	*N. vaccinii* DSM 43285^T
GEN III Biolog microplate tests
Sugars:
D-Cellobiose, D-galactose, β-gentiobiose, D-maltose, D-mannose, D-raffinose, L-rhamnose, sucrose, D-trehalose, and D-turanose	+	-
Polymers:
Dextrin, pectin	+	-
Tolerance to:
Lithium chloride, guanidine hydrochloride, sodium bromate, sodium chloride (4% and 8%), tetrazolium blue, and tetrazolium violet	+	-
Sugar alcohols:
D-Arabitol	+	-
D-Mannitol	-	+
Oxidation of amino acids:
L-Alanine, L-serine	+	-
L-Aspartic acid	-	+
Resistant to:
Fusidic acid, lincomycin, minocycline, troleandomycin, and vancomycin	+	-
Sugar acids:
L-Galactonic acid-γ-lactone, andD-galacturonic acid	+	-
Organic acids
γ-amino-n-Butyric acid, α-hydroxy-butyric acid, L-lactic acid, D-lactic acid methyl ester, p-hydroxy-phenylacetic acid, methyl pyruvate, and quinic acid,	+	-
α-keto-Glutaric acid, L-malic acid, mucic acid, propionic acid, and bromo-succinic acid,	-	+
API ZYM tests:
Alkaline phosphatase, α-chymotrypsin, cystine aminopeptidase, esterase (C4), esterase lipase (C8), lipase (C14), trypsin, and valine aminopeptidase	-	+
Other tests:
Niacin	+	-

+, positive result; -, negative result
Both strains reduced potassium tellurite and gave positive results for acid phosphatase, β-galactosidase α-glucosidase; oxidized, D-glucose, D-gluconic acid, glycerol, and myo-inositol (sugars); acetic acid, acetoacetic acid, butyric acid, β-hydroxy-butyric acid, α-keto-butyric acid, citric acid, and D-saccharic acid, (organic acids); L-arginine, L-glutamic acid and D-serine #2 (amino acids); were resistant to nalidixic acid, and rifamycin sv (antibiotics); and grew in presence of potassium tellurite, and Tween 40 (inhibitory compounds); and in the presence 1% sodium lactate, sodium chloride (1%) (salts) and at pH 5- 6. The strains gave negative results for arylsulfatase after 3 days, D-fucose, L-fucose, D-fructose, D-fructose-6-phosphate, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-glucose-6-phosphate, glucuronamide, 3-O-methyl-D-glucose, β-methyl-D-glucoside, α-D-lactose, D-melibiose, D-salicin, D-sorbitol, and D-stachyose (sugars); D-gluconic acid, D-malic acid, N-acetyl-neuraminic acid, and L-pyroglutamic acid (organic acids), D-aspartic acid, glycine-proline, L-histidine, and D-serine #1 (amino acids); inosine (nucleoside); aztreonam (antibiotic); gelatin (polymer), niaproof, and sodium formate (inhibitory compounds); α-fucosidase; α-galactosidase, N-acetyl-β-glucosaminidase; β-glucuronidase, and α-mannosidase (API-ZYM tests)

ISSN: 1471-2164