Table 2 Primers used in this study
From: A unique 15-bp InDel in the first intron of BMPR1B regulates its expression in Taihu pigs
Primer | Sequence (5′-3′) | Annealing temperature | Product size |
|---|---|---|---|
GAPDH-F | GGGCATGAACCATGAGAAGT | 56 °C | 229 bp |
GAPDH-R | AAGCAGGGATGATGTTCTGG | 56 °C | |
BMPR1B-T1-F | CCAGGTGATACTGATGTGC | 56 °C | 77 bp |
BMPR1B-T1-R | CAGTGGTGGTGGCATTT | 56 °C | |
BMPR1B-T3-F | GCGGGTTTCAGAGTGGTCAAG | 56 °C | 94 bp |
BMPR1B-T3-R | TGTATGGCAGGCTTGCTCCTTT | 56 °C | |
BMPR1B-T4-F | AACCAGTTGTCCCTGAGCTATGA | 56 °C | 132 bp |
BMPR1B-T4-R | GGTGGCATTTACATCGCAAGA | 56 °C | |
BMPR1B-GSP | GATTACGCCAAGCTTGGACAGTGGTGGTGGCATT | Â | Â |
15-bp-InDel-F | CACAGACCAGCTTGTCTTGAA | 56 °C | 242 bp |
15-bp-InDel-R | GGGCTCCTGTCTTTCTCTCT | 56 °C |