Fig. 1

Stimuli activate shared and distinct pathways of the innate immune response. A Across 3 biological replicates, ncgRNA (grey) and PAF1 KO (teal) cells were subject to a 3-hour stimulation by the treatments described. Following, RNA was extracted, purified, and sequenced. Aligned reads underwent DESeq2 differential gene expression analysis. B A heatmap of log2 foldchanges (ncgRNA stimulation / ncgRNA mock) for immune response genes (GO:0002376) confirms immune activation. Hierarchal clustering using Ward’s method identified notable clusters of genes for labeling. C GSEA was performed on gene expression data in ncgRNA cells for each stimulation, wherein genes were ranked by a weighted statistic for log2 foldchange and adjusted p-value. The normalized enrichment scores (NES) for, at most, the top 10 enriched Reactome pathways were plotted for each stimulus (p.adj < 0.05). The full GSEA output is available in Table S2A