Fig. 1

Genetically modified enteroids exhibit mutation-specific variation in miRNA expression. A Diagram illustrating how enteroid models were generated (Created with BioRender.com). B Principal component analysis (PCA) plot generated using miRNA expression profiles from Apc (A; n = 4), Apc/Kras/p53 (AKP; n = 1), Ctnnb1 (B; n = 5), Ctnnb1/Kras/p53 (BKP; n = 1), Kras/Rspo3/p53 (KRP; n = 2), Kras/Rspo3/p53/Smad4 (KRPS; n = 4), Kras/Rspo3/Smad4 (KRS; n = 3), Rspo3 (R; n = 2) mutant enteroids, and wild-type (WT; n = 3) controls. C Z-score of miRNA abundance for the 12 modules of miRNA expression, each with greater than 5 miRNAs in the module, as defined by DEGReport. Only miRNAs with baseMean > 500 and p-adj < 0.05 following DESeq2 likelihood ratio test (LRT) were included in the analysis. D-F Heatmaps show the magnitude of change in miRNA expression relative to WT by subtracting rlog normalized miRNA expression for each enteroid sample by the rlog average WT expression. Heatmaps shown are for Group K, Group E and Group F as defined by DEGReport. Color intensity shows the difference between rlog normalized miRNA expression and average WT. Color scale minimum saturates at -3 and maximum saturates at 3