Fig. 1

Profiling of differentially expressed genes during 12 key developmental stages of Arabica somatic embryogenesis (SE). A Heatmap generated from the normalized counts of 10,384 DEGs over the 12 developmental stages. Rows correspond to DEGs and columns to the developmental stages. Normalized counts of each gene were transformed in order to follow a standard normal distribution. Positive expression values are in green and negative values in red. B Hierarchical clustering of the 12 SE developmental stages according to the similarities in their transcript profiles. Clustering was performed using Pearson’s correlation coefficient. Cluster probabilities were calculated via a multiscale bootstrap with a total of 1,000 iterations. Clustering yielded 7 major nodes: Leaf, Dedifferentiation, Primary callus, Embryogenic callus, Embryogenic cell clusters, Redifferentiation and Embryo