Fig. 7

qRT-PCR validation of selected genes involved in ascorbic acid (AsA) biosynthesis of lettuce. A: Black bars represent the relative expression determined via qRT-PCR (left y-axis) and grey bars represent the level of expression of the transcripts (right y-axis). The error bars indicate the standard errors of three biological and three technical replicates. B: Scatter plots show linear regression and R² between RNA-Seq and qRT-PCR in terms of log₂FC