Fig. 5

Expression profiles of BCO2 and miR-330 in wild-type rainbow trout (WTrt) and yellow mutant rainbow trout (YMrt). A and B Expression patterns of BCO2 and miR-330 at different developmental stages. (C and D) Expression patterns of BCO2 and miR-330 in different tissues. 1: Fertilized-stage; 2: 4-cell; 3: 16-cell; 4: Multi-cell; 5: Blastula; 6: Gastrula; 7: Neurula; 8: Somites; 9: Heartbeating; 10: 1 day post hatching (1 dph); 11: 10 dph; 12: 1 month post hatching (1 mph); 13: 2 mph; 14: 3 mph. Ds: Dorsal skin; Vs: Ventral skin; Dm: Dorsal muscle; Vm: Ventral muscle; Br: Brain; E: Eye; Gi: Gill; Mk: Midkidney; Hk: Headkidney; In: Intestine; Li: Liver; Sp: Spleen; He: Heart. Reference gene: β-actin and U6; n = 3 different independent samples. Capital alphabet superscripts were used to indicate the differences within WTrt, and lowercase alphabet superscripts were used to indicate the differences within YMrt, different letters represent significant difference (P < 0.05) (one-way ANOVA). The difference analysis in the same developmental stage or tissue between WTrt and YMrt is indicated by * and **, *: P < 0.05, **: P < 0.01 (student’s t-test)