Table 4 Details of NAC selected for the validation of their expression profiles
Gene Name | Primer Name | Primer Sequence(5’-3’) | AnnealingTemp(°C) | Product Size(bp) | Unigenes |
|---|---|---|---|---|---|
ScNAC 78 | s1_NAC1 F | ACGGGGTACAACTGAACTGC | 60 | 166 | Sample_1_c26124_g1_i2 |
s1_NAC1 R | CCAACTGTTGCAGTGCCTAA | 59.9 | Â | Â | |
ScNAC46 | s1_NAC3 F | GGCAGCTTCAGTTTCTTGCT | 59.8 | 182 | Sample_1_c28778_g2_i1 |
s1_NAC3 R | AGCGTGATGAACAACAGCAG | 60.1 | Â | Â | |
ScNAC55 | s1_NAC5 F | AGCAAGCAACAAGGGAAGAA | 60 | 201 | Sample_1_c28922_g1_i2 |
s1_NAC5 R | AGGACAAGGTCACCAGGTTG | 60 | Â | Â | |
ScNAc89 | s1_NAC6 F | CTGGATGTCGTCGTAGCTGA | 60 | 176 | Sample_1_c29565_g1_i2 |
s1_NAC6 R | AGTCGGAGATCGTGGACAAC | 60.1 | Â | Â | |
ScNAC36 | s2_NAC2 F | GCGGTCTGGACGGATAATAA | 59.9 | 214 | Sample_2_c29193_g1_i2 |
s2_NAC2 R | ACTGAAGCCTGAACGACGAT | 59.9 | Â | Â | |
ScNAC81 | s2_NAC4 F | CACAAACTCATCCGGCTACA | 59.7 | 195 | Sample_2_c22709_g2_i1 |
s2_NAC4 R | GAGCTGGAAGAACGACGAAC | 60 | Â | Â | |
ScNAC58 | s3_NAC2 F | TGTTTCCACATTGCTGGTGT | 60 | 150 | Sample_3_c42536_g1_i3 |
s3_NAC2 R | CCTTTTGGAGAGTGCTCTGG | 60 | Â | Â | |
ScNAC27 | s3_NAC3 F | AGTCGGAGATCGTGGACAAC | 60.1 | 170 | Sample_3_c41194_g1_i8 |
s3_NAC3 R | CTGGATGTCGTCGTAGCTGA | 60 | Â | Â | |
ScNAC69 | s5_NAC1 F | TAATCCGGCCGGTAGTAGTG | 60 | 158 | Sample_5_c29179_g1_i1 |
s5_NAC1 R | TTCTCCCTCTTGCATTGCTT | 60 | Â | Â | |
ScNAC34 | s5_NAC4 F | GGTGAACCAGTCGTTGTCCT | 60 | 228 | Sample_5_c29442_g1_i1 |
s5_NAC4 R | AAGGTGAAGGTGGAGAAGCA | 59.8 | Â | Â | |
ScNAC70 | s5_NAC6 F | CTTCGCAAAGAGGTGGAAAG | 60 | 209 | Sample_5_c16801_g1_i1 |
s5_NAC6 R | CTTTGGAGTTTTCGGACCAA | 60.1 | Â | Â | |
ScNAC7 | s5_NAC7 F | CCGGATCGATTAGTTGGTTG | 60.3 | 228 | Sample_5_c34885_g1_i2 |
s5_NAC7 R | CCAACCTGGAGCTACGAGAG | 60 | Â | Â | |
ScNAC59 | s5_NAC8 F | ATAAGGTGGCCACAGACTGG | 60 | 191 | Sample_5_c11486_g1_i1 |
s5_NAC8 R | GCTGTTGAGGAACAGCAATG | 59.4 | Â | Â | |
ScNAC80 | s6_NAC1 F | CACGACGTTGCCATTATCTG | 60.1 | 237 | Sample_6_c31836_g1_i1 |
s6_NAC1 R | GAAGGAGGCAGGCATTGTAG | 59.8 | Â | Â | |
ScNAC65 | s6_NAC4 F | TCTGCGAAACACAGGAGATG | 60 | 226 | Sample_6_c27451_g1_i3 |
s6_NAC4 R | CGCAATGCACAGGAGAGATA | 60 | Â | Â |