Fig. 11

Ingenuity pathway analysis of upstream transcriptional regulators of -omic changes in chronological order. A Heatmap of top IPA upstream transcriptional regulators that were activated (orange cells in the heatmap, representing positive z-scores) or inhibited (blue cells in the heatmap, representing negative z-scores) in both transcriptomic and proteomic measurement are shown. Expectedly, NFκB and STAT3 are potentially activated after radiation exposure, with inhibition of HDAC3 and the 26s proteasome. B Differential protein changes at day 1 (proteomics) compared with predicted transcriptional activity as measured by z-scores (range between -6.102 to 9.304) at following days (transcriptomic predictions) are shown. Regulators such as SRC, TGFbeta, NFATC2 and FN1 are increased at the protein level as well as predicted to be activated up to 1 week later by transcriptional changes