Fig. 4
From: Benchmarking datasets for assembly-based variant calling using high-fidelity long reads
Acquired variant detection rates of CLR and HiFi assemblies to estimate relative false-positive calling rates. A–D, Number of shared or strain-specific acquired variants detected in each assembly. Acquired variants were divided into four groups according to their size and type: A 5–49-bp deletion; B 5–49-bp insertion; C ≥ 50-bp deletion; and D ≥ 50-bp insertion. Blue bar represents acquired variants perfectly matched for position and size between ALT1 and ALT2 using the same sequencing mode. Red bar represents partially-matched acquired variants. Gray bar represents the remaining acquired variants identified in a single strain. E Overlap ratios for common acquired variants detected using CLR and HiFi assemblies. Full circle represents the ratio of CLR variants covered by HiFi and the empty circle represents vice versa. Colors represent partial- or full-matched variant sets