Fig. 1

Data analyses of RNA-seq samples. (A) Drawing of a P. canidia adult forewing showing the approximate location of the three dissected areas mapped to the wing. (B) P. canidia pupa (3 h old) with the three dissected tissues marked on the wing. (C) Principal component analyses of RNA samples clustered by the area of the wing of which samples were dissected. Apart from three outlier groups, the rest of the samples clustered first with others from the same wing location. Outlier groups were removed from downstream analyses. (Red: Wing tissues dissected from the Cu1 wing sector, Green: Wing tissues dissected from an area that is proximal to the spot pattern, and Blue: Wing tissues dissected from an area that contains the spot pattern. (D) Number of differentially expressed genes (DEGs) for the comparison of M3 “Spot” versus Cu1 “Control” tissue