Fig. 4

Final optical densities of MG1655, BM28 ΔlysU and BM28c (BM28 cured of pOF39) with and without pOF39 grown at high temperatures. Cultures were incubated at the indicated temperatures in a shaking water bath for 23 h, in quintuplicate, and their final optical densities were measured and recorded. Statistical comparisons between final optical densities of cells with and without pOF39 were determined using an unpaired t test with Welch correction, and P values > 0.05 are indicated with ns, ≤ 0.001 are indicated with ***, and ≤ 0.0001 are indicated with ****. Generated with GraphPad Prism 9.4.1