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Table 2 Cluster overview

From: Single-cell RNA sequencing of mid-to-late stage spider embryos: new insights into spider development

Cluster

Mega Cluster

Title

Tissue

I

A

Dorsal tissue, and ectoderm patterning of developing appendages (1)

Ectoderm

II

A

Ectoderm patterning of developing appendages (2)

Ectoderm

III

B

The developing central nervous system (CNS) (1) – Differentiating and differentiated neurons (1)

CNS

IV

A

Ectoderm patterning of developing appendages (3)

Ectoderm

V

B/C

The developing central nervous system (CNS) (2) – EMT-like processes in neural precursor determination?

CNS

VI

B

The developing central nervous system (CNS) (3)—Early differentiating neural cells (1)

CNS

VII

C

The developing mesoderm (1) – EMT-like processes in visceral mesoderm development?

EMT (Mesoderm)

VIII

B

The developing central nervous system (CNS) (4)—Early differentiating neurons?

CNS

IX

–-

Opisthosomal appendages

Ectoderm

X

B

The developing central nervous system (CNS) (5): Early differentiating neural cells (2)

CNS

XI

B

The developing central nervous system (CNS) (6)—Neural precursors

CNS

XII

B

The developing central nervous system (CNS) (7) – Differentiating and differentiated neurons (2)

CNS

XIII

A

Ectoderm patterning of developing appendages (4)

Ectoderm

XIV

B/C

The ventral sulcus (VS)

Ventral Sulcus

XV

A

Ectoderm patterning of developing appendages (5)

Ectoderm

XVI

B

The peripheral nervous system (PNS)

PNS

XVII

B

The developing central nervous system (CNS) (8)—Early differentiating neurons

CNS

XVIII

A/C

Ectoderm patterning of developing appendages (6): EMT-like processes in the appendage epithelium

EMT (Ectoderm)

XIX

B

The developing central nervous system (CNS) (9) – The sensory nervous system of the head

CNS

XX

–-

The developing heart

Mesoderm

XXI

 

Midgut development, yolk metabolism, hematopoiesis, and immune response

Yolk + Blood cells /Endoderm

XXII

–-

The stomodaeum and the ventral midline

Ectoderm

XXIII

–-

Midgut development and yolk metabolism

Yolk cells /Endoderm

XXIV

B

A mini-cluster consisting of only 5 cells that suggests a function in leg-innervation

PNS

  1. Mega Cluster A: Ectoderm patterning of developing appendages
  2. Mega Cluster B: The developing central nervous system (CNS)
  3. Mega Cluster C: Epithelial-to-mesenchymal transition (EMT)