Table 2 The sequences information of primers used for RT-qPCR
DEGs | Gene name | Primer sequences (5’ to 3’) | Product size (bp) | Amplification efficiency (%) |
|---|---|---|---|---|
Estradiol 17-beta-dehydrogenase | 17β-HSD | F: TCCTCTTTCTCACACGCTTTCTCTC | 85 | 99.2 |
R: AAGTCTGCCAAGTTCCTCATCTACC | ||||
Estrogen receptor | ER | F: TGCCTCCTACCAATACTGCTGAAG | 110 | 95.4 |
R: GCTATGCTACGCCTGCTCACC | ||||
Progesterone receptor | PR | F: ACTGTGTTTGACTGGGCAAGAAATG | 126 | 97.7 |
R: CGTGGAGTTGAGCATTGGACATTC | ||||
Growth hormone | GH | F: AAATCCGTGTTGGTGATGGTCAAAC | 126 | 101.8 |
R: AGTCAGTTCCACAGTGTAGCAGTTC | ||||
Oocyte meiosis | OM | F: GGTGAGACGCCCATCCTTTCC | 141 | 98.5 |
R: TCGTTTCCTCAATCCTCCCTGTTC | ||||
Vitellogenesis | VGS | F: TGCCGTCACCTCCCTCTGTC | 118 | 97.3 |
R: GCGAGATGCCAGCCTAGTAGAAC | ||||
Neuropeptide F receptor | NFR | F: CGCTGCTGTTGTTGGTGTATCTG | 83 | 101.4 |
R: CCTTGGACACTAGGTAGCCGATTC | ||||
Neuropeptide Y receptor | NYR | F: TTAACCTGCCTCTGTGCGATGG | 120 | 102.6 |
R: GAGAAACGGCGAACTGCTTGTG | ||||
|  | β-actin | F: GACTCCTACGTAGGAGACGA | 235 | 98.6 |
R: CGTTGAAGGTCTCGAACATGA |