Fig. 4

pyramus is a putative novel cell type-specific target of Pnt. (A) Genomic track showing a prominent Pnt ChIP-seq peak about 11 kb 3’ of pyramus (pyr). (B) Larval eye disc scRNA-seq plot showing the expression (blue) of pyr in R1/6, R7 and the Convergence cell clusters. (C) snATAC-seq genomic tracks at the pyr locus show prominent peaks in R1/6, R7 and the Convergence cell clusters. This peak is present at the same genomic location as the 3’ peak in the ChIP-seq data sets (A). The black box represents the peak-region DNA that was tested for enhancer activity in vivo. (D-F) Immunostaining of transgenic larval eye discs carrying the peak-region DNA in (A) and (C) driving expression of a dGFP reporter. Larval eye discs were stained with GFP (E) and Runt (F), which marks R7 and R8. Many ommatidia show coexpression of GFP and Runt antibodies (D)