Fig. 4

Proteins from the “hypothetical protein” cluster share a signal peptide, membrane-bound protein domain and significant similarity to plexin domain-containing proteins. A) Dendrogram of the amino acid alignment of proteins from the “hypothetical protein” cluster with corresponding protein domain annotation and chromosomal location to the right of figure. Asterisks next to gene identifier (Smp…*) denote non-subtelomeric gene copies. Red circles at nodes indicate an ultrafast bootstrap value < 70. Scale bar indicates number of nucleotide substitutions per codon. B) Multiple sequence alignment of selected hypothetical proteins where coloured residues indicate > 80% identity. Domains identified by InterProScan (blue and red) were trimmed for clarity, and the Plexin Semaphorin Integrin domain (green) inferred by hmmsearch was overlaid. C) Expected distance error (Ångströms) of one protein from the cluster (Smp_173350.1) using Alphafold with domains overlaid. D) Corresponding 3D protein model coloured according to the identified domains representative of this cluster