Metatranscriptome analysis of symptomatic bitter apple plants revealed mixed viral infections with a putative novel polerovirus

Table 1 PCR primers designed to detect and discriminate viruses in this study

Primer name	Sequence (5´ to 3´)	Position	PCR product size (bp)	Annealing temperature (^oC)
BaABYV -F	TGA TCG CGA ACT ACA TGT CC	3236–3255	939	56
BaABYV -R	GGA ACT GCC GTC TAC CTA TTT	4174 − 4154	939	56
SqVYV-F	GGA AGC ACT CAT GCC TGA TATT	8517–8538	1158	55
SqVYV-R	GGT GCT GAA CAG TAC CTC A	9674 − 9656	1158	55
CiaRV-F	TCT CCG GTA TTT GAG AAG CAC	10,973–10,993	641	52
CiaRV-R	CCG AGC AAC CCT TAT CTG TTT′	11,613 − 11,593	641	52
WMV F	GAA TCA GTG TCT CTG CAA TCA GG	2703–2725	822	55
WMV R	ATT CAC GTC CCT TGC AGT GTG	3524 − 3504	822	55

BaABYV (Bitter apple aphid-borne yellows virus), SqVYV (Squash vein yellowing virus), CiaRV (Citrus-associated rhabdovirus), WMV (Watermelon mosaic virus)

ISSN: 1471-2164