Table 2 Sequencing information and assembly statistics for BF5_0283 Trycycler and Mt009 Trycycler, Unicycler and metaFlye assembly
| Â | BF5_0283 | Mt009 | ||
Sequencing information | Â | Â | ||
ONT library size a | 1,022,088 | 685,827 | ||
ONT mean read length [b] a | 1887.3 | 1910.9 | ||
Illumina library size (pair-end) | 2 × 27,521,418 | 2 × 23,515,083 | ||
Illumina mean read length [b] | 75 | 75 | ||
Assembly method | Trycycler | Trycycler | Unicycler | metaFlye |
Description | Long-read assembly (followed by long and short-read polishing) | Long-read assembly (followed by long and short-read polishing) | Short-read-first hybrid assembly (short read assembly, followed by long-read plus contig assembly) and polishing | Long read assembly |
Total length of assembly (bp) | 6,034,252 | 7,658,097 | 17,479,769 | 19,919,184 |
Total number of contigs in assembly | 3 | 3 | 21 | 188 |
Number of contigs in assembly (> = 50,000 bp) | 3 | 3 | 9 | 17 |
Average depth of ONT sequencing coverage a | 319.7x | 171.1x | 75.0x | 65.8x |
Average depth of Illumina sequencing coverage | 342.1x | 230.3x | 100.1x | 88.6x |
Reference coverage (%)b | Â | Â | Â | Â |
Vibrio campbellii ATCC BAA 1116 | 71.49% | 71.41% | 71.55% | 71.55% |
Enterovibrio norvegicus Alg239-V16 | 0.04% | 24.07% | 82.87% | 82.90% |
Klebsiella pneumoniae KCTC 2242 | 0.01% | 0.01% | 93.39% | 90.61% |