Fig. 1

Overview of sample collection and RNA-seq data analysis. (A) This picture shows chicken embryos at developmental stages HH17, 19 and 21. The precursor region of chicken wings and breast, which was collected for RNA-seq analysis, is indicated by the dotted rectangle. A cross-section of the embryo body is also provided to better understand the structures developing at this axial level. (NT) neural tube, (NO) notochord, (G) midgut, (LB) limb bud, (SO) somite and (DR) dorsal root ganglion. (B) The flowchart illustrates the key steps involved in RNA-seq data analysis. Boxes in the flowchart represent specific analysis stages, while arrows depict the flow of data between them