Fig. 1

Study design. Heterozygous MIR484 knockout HEK293 cell line (HEK293 MIR484 KO^het) and homozygous MIR185 knockout HEK293 cell line (HEK293 MIR185 KO^hom) were used as models of CNV-affected miRNAs in CAKUT. Cell lines were treated simultaneously with the corresponding miRNA mimics and NT miRNA mimic. Two of the investigated genes (MDMD2 and PKD1) have been selected as targets of both hsa-miR-484 and hsa-miR-185-5p mimics. Additional three candidate target genes with a potential role in CAKUT have been selected for each miRNA. As candidate target genes of hsa-miR-185-5p RHOA, ATF6 and CDC42 have been selected, while NOTCH3, FIS1 and APAF1 have been chosen as candidate target genes of hsa-miR-484. Both miRNA mimic treatments have been performed on HEK293 WT cell lines as well. Expression of target genes for miRNA mimic treatments have been compared to the corresponding NT miRNA treatments to explore the responsiveness of the expression of target genes to treatment with hsa-miR-484 and hsa-miR-185-5p mimics