A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT

BMC Genomics

Table 1 Expression of hsa-miR-484 and hsa-miR-185-5p target genes after treatment of the cell lines with corresponding miRNA mimics

Genes	Effect of		Effect of		Effect of		Effect of
	hsa-miR-484 mimic on		hsa-miR-185-5p mimic on		hsa-miR-484 mimic on		hsa-miR-185-5p mimic on
	HEK293 MIR484 KO^het		HEK293 MIR185 KO^hom		HEK293 WT		HEK293 WT
	cell line		cell line		cell line		cell line
	FC	P^avalue	FC	P^a value	FC	P^a value	FC	P^a value
MDM2	1.21	0.377	1.13	0.563	0.69	0.057	0.60	0.019
PKD1	1.14	0.759	0.79	0.496	1.31	0.164	0.74	0.306
NOTCH3	0.82	0.035	N/A	N/A	0.75	0.003	N/A	N/A
FIS1	1.01	0.940	N/A	N/A	1.29	0.032	N/A	N/A
APAF1	1.86	0.198	N/A	N/A	1.4	0.235	N/A	N/A
RHOA	N/A	N/A	1.39	0.03	N/A	N/A	1.16	0.055
ATF6	N/A	N/A	1.88	0.007	N/A	N/A	1.77	0.008
CDC42	N/A	N/A	0.69	0.107	N/A	N/A	0.83	0.169

Data marked in bold represents statistically significant differences in mRNA expression between miRNA mimic treated cells and NT mimic treated cells. FC–fold change in mRNA level after miRNA mimic treatment compared to NT mimic treatment; ^aStudent’s ttest
^aStudent’s ttest

ISSN: 1471-2164