Fig. 4

Identification of cell type-specific regulons by SCENIC analysis and prediction of target transcription factors (TF) within downstream genes (colored in Fig. 3G) using the CHEA Transcription Factor Targets Dataset in Harmonizome 3.0. Integration of the four datasets is processed and clustered into three cell-specific types (A). TFs specific to SV single-cell subpolulation are selected with calcRSS function in SCENIC (B). The representative TFs of the SV single-cell subpopulation are confirmed in the gEAR database (C) and integrated datasets (D). These genes (vertical level) clustered in the oxidative phosphorylation and electron transport chain pathways are predicted to be regulated by these TFs (horizontal level) using the CHEA Transcription Factor Targets Dataset (E). These TFs with red stars are also found in our SCENIC analysis results (Additional file 5: Fig. S5)