Fig. 8

The validation of the critical genes. A The gene TPM counts of candidate critical genes. B The relative mRNA expression levels of FOXO3 and PLIN1 in the liver by qRT-PCR. C The ROC validation for critical genes. NS p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: LCu = the low-Cu feeding group; LCG = the control group for the low-Cu feeding period; SCu = the high-Cu supplement group; SCG = the control group for the high-Cu feeding period; TPM = transcripts per kilobase million reads; qRT-PCR = quantitative real-time polymerase chain reaction; ROC = the receiver operating characteristic curve