Fig. 6

Aedes aegypti mounts a distinct response to fungal pathogens. Principal component analyses (PC1 vs PC3) of transcriptomes from Ae. aegypti (A) and Anopheles gambiae (s.l.) (B) combining data from two separate RNAseq experiments. From experiment #1: unchallenged (UC) mosquitoes and mosquitoes challenged with Escherichia coli (Ec), Erwinia carotovora carotovora 15 (Ecc15), Serratia marcescens type strain (Sm), Providencia rettgeri (Pr), Staphylococcus aureus (Sa), Micrococcus luteus (Ml), Enterococcus faecalis (Ef), Saccharomyces cerevisiae (Sc), and Candida albicans (Ca). From experiment #2: unchallenged (UC) mosquitoes, and mosquitoes challenged with Beauveria bassiana (Bb), Metarhizium anisopliae (Ma), and concentrated (OD₆₀₀ = 100) heat-killed (HK) Ec, Ef, and Ca; all data are from 12-hour timepoint. Principal component analyses (PC1 vs PC2) of transcriptomes from Ae. aegypti (C) and An. gambiae (D) challenged with concentrated HK Ec, Ef, and Ca; all data are from 12-hour timepoint. Criteria for inclusion in the bacterial and fungal cores, with Venn diagrams showing the size and relationship between cores in Ae. aegypti and An. gambiae (E). Plots depicting the expression (in transcripts per million) of genes in the bacterial and fungal cores in Ae. aegypti (F-K) and An. gambiae (L-Q) juxtaposing the response to Ca versus Ecc15 (F, L), Ca versus Ml (G, M), Ma versus Ec (I, O), Ma versus Ef (J, P), HK Ca versus HK Ec (H, N), HK Ca versus HK Ef (K, Q); all data are from 12-hour timepoint except Ma which is from 36-hour timepoint. Clustering analyses of bacterial and fungal core genes in Ae. aegypti (R), and An. gambiae (S) comparing log₂FC (calculated by DESeq2) in insects challenged with Ec, Ecc15, Sm, Pr, Sa, Ef, Ml, Ca, HK Ca, HK Ec, HK Ef (12-hour), and with Bb and Ma (36-hour). Volcano plots depicting genes that are differentially expressed (DESeq2 fold-change ≥1.5, padj < 0.05) in a comparison of HK Ec- and HK Ef-challenged versus HK Ca-challenged Ae. aegypti (T) and An. gambiae (U); all are data from 12-hour timepoint