Fig. 4

DPPA3 acts in the downstream of PRDM14 and independently of TET1. (A) ChIP-seq data showing a PRDM14 peak in an upstream region of Dppa3 and enrichment of histone marks typical of an active enhancer. Chromatin accessibility data is also shown. Published ChIP-seq data from ESCs and EpiLCs overexpressing Prdm14 [40, 41] and from E13.5 female PGCs [34] were reprocessed. (B) Dppa3 expression in E7.25-E7.5 Prdm14 KO PGCs revealed by single cell microarray analysis. (C) Violin plots showing that CG hypermethylation of Dppa3 KO PGCs is already seen at E11.5 (10-kb windows, n = 14,395) (left) while hypermethylation of Tet1 KO PGCs occurs only after E12.5 (n = 6,806) (right). Horizontal bars indicate the median values. Published WGBS data [11] were retrieved and reprocessed for control and Tet1 KO female PGCs. (D) Heatmap showing that different regions are hypermethylated in Dppa3 KO and Tet1 KO E13.5 female PGCs (n = 166,566). The heatmap was produced based on the extent of CG methylation differences (∆CG methylation). Published WGBS data [14] were retrieved and reprocessed for control and Tet1 KO PGCs