Fig. 1

RNA-seq analysis of differential gene expression in response to HMGB1-OE. A HMGB1 expression in HeLa cells after transiently transfected with HMGB1 overexpression or control vector, as determined by RT-qPCR. B HMGB1 overexpression and control vector followed by Western blot using antibody against FLAG. Three replicates. C Immune fluorescence experiment showing the subcellular localization of HMGB1 and HMGB1-Flag in HeLa cells. D HMGB1 expression values quantified using RNA sequencing data. E Detection of the HMGB1 regulated genes on the volcano plots, up DEGs (FC ≥ 2, FDR < 0.05) are labeled red, whereas down DEGs (FC ≤ 0.5, FDR < 0.05) are labeled blue. (F) Hierarchical clustering of DEGs in control and overexpression cells (Expression values are log₂-transformed and the median-centered by each gene)