Fig. 5

HMGB1 binds snoRNAs and scaRNAs with high affinity and affect rRNA modification. A Bar plot represent for the indicated Top-100-bound RNA biotypes in the IP and Input samples. (A cut off of IP/input fold change value ≥ 2 and p-value < 0.05). B Scatter plot showing comparison between expression profiles of IP (x axis) and Input (y axis) (Expression values are log10-transformed). snoRNAs are colored in red, scaRNA are colored in green, others are in grey. C The top 20 enriched snoRNA enriched in HMGB1 IP sample (p-value < 0.05). D The distribution of reads across the whole region in the snoRA63 and snoRD22 genomic location and RIP-qPCR validation of HMGB1-bound snoRNA. And validation of HMGB1-bound scaRNA by RIP-qPCR. E Bar plot showing the uvRIP-qPCR results for four snoRNAs/scaRNAs that were bound by HMGB1