Fig. 6

HMGB1 overexpression decreased the methylation modification of 28S rRNA and promote rRNA transcription and protein synthesis. A The 28S-FU2-RT/FD1-RT signal intensity ration detected by RT-qPCR in HMGB1-OE (left panel) and HMGB1-KD (right panel samples). n = 3 biological replicates. LD: low dNTP; MD: middle dNTP; HD: high dNTP. B RT-qPCR showing the increase of rRNA levels after HMGB1 overexpression. n = 3 biological replicates. C Representative histogram showing the HPG fluorescent staining signal result in the protein synthesis activity in HMGB1-OE and Ctrl cells. Three replicates. D Representative histogram showing the HPG fluorescent staining signal result in the protein synthesis activity in HMGB1-KD and Ctrl cells. Three replicates. E Diagram depicting a simplified model of HMGB1 influence on ribosome modification, rRNA expression and protein synthesis