Fig. 7

Binding power and overexpression analysis of key transcription factors with EsFPS, EsSS, and EsSE promoters. A: Electrophoretic mobility shift assay analysis of crucial transcription factor binding to EsFPS, EsSS, and EsSE promoters; B: Molecular docking of EsMYB-r1 with unmethylated EsFPS; C: Molecular docking of EsMYB-r1 with methylated EsFPS; D: Molecular docking of EsCAMTA1 with unmethylated EsFPS; E: Molecular docking of EsCAMTA1 with methylated EsFPS; F: Gene expression after EsMYB-r1 overexpression; G: Gene expression following EsB3-ARF1 overexpression; H: Gene expression following EsCAMTA1 overexpression; and I: triterpene saponin content following overexpression of sensitive transcription factor genes. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001. Note: 1–4, 13–16, and 25–28: promoters of EsFPS; 5–8, 17–20, and 29–32: promoters of EsSS; 9–12, 21–24, and 33–26: promoters of EsSE