Fig. 2

Depletion of PDPK1 using the dTAG method reduces expression of mitotic genes. (a) Western blot showing PDPK1 expression in parental CHP-134 cells and cells engineered so that endogenous PDPK1 is expressed in frame with a FKBP12(F36V)-2xHA tag, treated with either DMSO or 500 nM dTAG47 (D47) for 24 h to induce PDPK1 degradation. Uncropped blots are presented in Additional File 3: Figure S1. (b) mRNA analysis of genes involved in mitosis following treatment of engineered CHP-134 cells for 24–48 h with 500 nM dTAG47. GAPDH is used for normalization as a reference gene (n = 3 biological replicates, error bars are standard error, ***P < 0.001, **P < 0.01, *P = 0.05 using unpaired t-test, two-tailed)