Fig. 2

SSB hotspots are significantly enriched around TSSs in different tissue types. (A) Aggregate plots of the normalized density scores (Y-axes) of the positions of all SSBs and various SSB hotspots within ± 5,000 bp of TSSs in heart. The opaque vertical rectangles represent the ± 200 bp regions around the TSSs. The signal from the template and nontemplate strands is shown respectively above and below the zero lines. (B, C) The TSS-SSBs enrichment ratios (Y-axes) for all SSBs (background) and SSB hotspots on either template or non-template strands for different tissues: heart and liver (B) and the other four tissues (C). Yellow represents background (all SSBs), red represents sample-level hotspots (depth ≥ 2), blue represents sample-shared hotspots (depth ≥ 1) and purple represents sample-shared hotspots (depth ≥ 2). Note, two data points in sperm for the sample-shared hotspots (depth ≥ 1) and background have the same values (Supplementary Table 3), thus only the latter is shown