Fig. 3

SSB hotspots are enriched on the template strand in the immediate vicinity of TSSs. (A) Aggregate plots of the normalized density scores (Y-axes) of the positions of all SSBs or sample-shared hotspots (depth ≥ 1) within ± 200 bp of the TSSs for heart (A). The signal from the template and nontemplate strands is shown respectively above and below the zero lines. (B, C) Odds ratios (Y-axes) of enrichment of sample-shared SSB hotspots (depth ≥ 1) found on other template or non-template strands in the indicated non-overlapping distance bins to TSSs in the different tissues (X-axes). Due to the differences in the magnitudes of the odds ratios, the Y-axes in (B) and (C) have different scales. (D) The template vs. nontemplate ratios (Y-axes) of SSB hotspots in each distance and each tissue. (B–D) The red dashed horizontal lines represent odds ratios (B, C) or template vs. nontemplate ratio of 1 that represent respectably no enrichment or no strand preference. See Supplementary Table 3 for the exact numbers and Methods for more details